RT-LAMP assay

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Original Article
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Reporting a visual detection of SARS-CoV-2 by a rapid, simple, sensitive, and specific RT-LAMP method with the eight primer sets. One major limitation of this assay is the designing of primers for effecient LAMP RT-PCR results.
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 		33098286
(Am J Trop Med Hyg)
PMID
33098286
Date of Publishing: 2020 Dec
Title A sensitive reverse transcription loop mediated isothermal amplification assay for direct visual detection of SARS-CoV-2
Author(s) nameLau YL, Ismail IB et al.
Journal Am J Trop Med Hyg
Impact factor
2.25
Citation count: 4

In this study, a colorimetric RT-LAMP method targeting the RdRp gene is developed for detecting SARS-CoV-2 within 60 min. The results showed high sensitivity and specificity of 95.74% and 99.95%, respectively. The assay result is comparable to that of commercial qRT-PCR assays implying its beneficial use as a diagnostic method for COVID-19 screening.
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 		33165486
(Analyst)
PMID
33165486
Date of Publishing: 2020 Nov 9
Title Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Author(s) nameNawattanapaiboon K , Pasomsub E et al.
Journal Analyst
Impact factor
3.98
Citation count: 20

Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay using extracted RNA in comparison with commercial Corman's LightMix E gene RT-PCR as reference. Variplex RT-LAMP assay showed a lower sensitivity, compared to commercial E gene RT-PCR, but sensitivity increased (86.4%) when samples with E gene Ct values <35 were considered.
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 		32891938
(J Clin Virol)
PMID
32891938
Date of Publishing: 2020 Nov
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R et al.
Journal J Clin Virol
Impact factor
2.95
Citation count: 33

Evaluating the performance of CE-labeled variplexTM real-time SARS-CoV-2 RT-LAMP assay from direct respiratory samples, without RNA extraction, and comparing it with VIASURE and Allplex RT-PCR assays. The Allplex RdRP assay showed the highest sensitivity (84 %), followed by E and N gene tests from the same kit. When variplex RT-LAMP assay was combined with that of other diagnostic assays under analysis, the sensitivity was increased to 92 and 100 %. Ct values of RT-LAMP showed a weak correlation with RT-PCR, Pearson coefficients ranging from (0.44-0.47)
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 		32891938
(J Clin Virol)
PMID
32891938
Date of Publishing: 2020 Nov
Title Use of the variplex SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis
Author(s) nameRödel J, Egerer R et al.
Journal J Clin Virol
Impact factor
2.95
Citation count: 33

Reporting a rapid, highly sensitive, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the S gene for SARS-CoV-2 detection. Assay sample to result time is 55 min; Assay result is qualitative (visually purple to blue)
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 		32848011
(mSphere)
PMID
32848011
Date of Publishing: 2020 Aug 26
Title Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection
Author(s) nameHu X, Deng Q et al.
Journal mSphere
Impact factor
4.28
Citation count: 27

In this study, researchers tested a rapid, isothermal, colorimetric RT-LAMP protocol by using a specific primer set based on the N gene for detecting SARS-CoV-2. The assay detected SARS-CoV-2 RNA, with a detection limit corresponding to a CT value of 30 for RT-qPCR, with a sensitivity of 97.5% and a specificity of 99.7%. RT-LAMP assay was also tested with the ORF1a primer set. ORF 1a primer set was found to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT 25, when compared with E gene RT-qPCRderived CT values.
Primer sequences used in the study are taken from Zhang et al., (2020). And the details are provided in Supplementary Tables (Table S1).
The researchers have developed a multiplexed sequencing protocol (LAMP-sequencing) to analyze the sequences of many RT-LAMP reaction products.
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 		32719001
(Sci Transl Med)
PMID
32719001
Date of Publishing: 2020 Aug 12
Title A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Author(s) nameDao Thi VL, Herbst K et al.
Journal Sci Transl Med
Impact factor
11.64
Citation count: 203

In this study, researchers have developed a rapid, isothermal method, a swab-to-RT-LAMP assay that can be performed without RNA extraction, for detecting SARS-CoV-2. The assay had a specificity of 99.5% and sensitivity of 86% (for CT < 30). Some positive samples did not show a color change but did so when repeated for a second time. Therefore it is recommended to run this assay using technical duplicates.
Primer sequences used in the study are taken from Zhang et al., (2020). And the details are provided in Supplementary Tables (Table S1).
The researchers have developed a multiplexed sequencing protocol (LAMP-sequencing) to analyze the sequences of many RT-LAMP reaction products.
✍
 		32719001
(Sci Transl Med)
PMID
32719001
Date of Publishing: 2020 Aug 12
Title A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Author(s) nameDao Thi VL, Herbst K et al.
Journal Sci Transl Med
Impact factor
11.64
Citation count: 203

Reporting a simple, rapid, and visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SARS-CoV-2 by targeting the N gene. The results were observed using a real-time PCR machine or by a colorimetric change from red to yellow. The results showed 92.9% concordance with the RT-qPCR assay. When the template input is more than 200 copies per 25 microL reaction, the RT-LAMP assay requires 30 min for real-time fluorescence monitoring or 40 min for visual detection reaction to complete
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 		32325642
(Int J Mol Sci)
PMID
32325642
Date of Publishing: 2020 Apr 18
Title A Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
Author(s) nameLu R, Wu X et al.
Journal Int J Mol Sci
Impact factor
4.21
Citation count: 88